Most Expected Questions of Life Science: Nitrogen Metabolism in Plants!

By Renuka Miglani|Updated : December 22nd, 2021

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As we all know, the CSIR-NET exam is very near and there is no time left for revision. At this point of time, you must be seeking some ready-made authentic short revision notes or some important questions to practice just before the exam. How will you react if you get all these study materials at one place? It will be beneficial for you at this time right! We at BYJU'S Exam Prep comes up with the Most Important Questions of Nitrogen Metabolism in Plants for Life Sciences to help you score good marks which have been meticulously designed by our experienced subject-matter experts.

So what are you waiting for? Scroll down the article below and start study for the CSIR NET Exam, these questions will be fruitful during last-minute revision to fetch more marks in the exams. Students can also download it as a PDF file and save it for future purposes.

Most Important Questions of Nitrogen Metabolism in Plants (Download PDF)

1. Match the following and choose the correct option.

I) Frankia                     A). Soyabean

II) Anabena                  B). Azolla

III) Brady rhizobium      C). Casuarina



  1. I-A, II-B, III-C 
  2. I-C, II-B, III-A 
  3. I-B, II-C, III-A 
  4. None 

2. In soil, by the addition of nitrogen containing fertilizer, gas exchange process is affected. Which of these is true statement regarding this.

  1. It causes production of antibiotic in bacteria which leads antibiotic resistance. 
  2. This addition results in release of NO and N2O which is greenhouse gas 
  3. It causes consumption of methane gas. 
  4. It causes production of methane gas. 

3. Match the following and choose the correct option:

I) Conversion of organic nitrogen into ammonia                          A). Nitrification

II) Conversion of Nitrite or nitrate into atmospheric nitrogen        B). Ammonification

III)Conversion of atmospheric nitrogen into ammonia                  C). Nitrogen fixation

IV) Conversion of ammonium ion into nitrite or nitrate                 D). Denitrification



  1. I-B, II-D, III-C, IV-A 
  2. I-D, II-B, III-C, IV-A 
  3. I-B, II-C, III-D, IV-A 
  4. I-B, II-D, III-A, IV-C 

4. Root nodules of legume plants contains leghaemoglobin, what is its function?

  1. Regulate oxygen supply. 
  2. Regulate growth of nodules. 
  3. Regulate denitrogenase activity. 
  4. Regulate the expression of nif gene. 

5. During biological nitrogen fixation, how many ATP molecules are required for the conversation of one nitrogen into two ammonium ions?

  1. 16 ATP 
  2. 12 ATP 
  3. 10 ATP 
  4. 8 ATP 

6. Regarding the conversion of NO3 - into NO2- which occurs in plant cell, following statement are given:

I. This conversion is catalysed by nitrate reductase.

II. This conversion occurs in cytosol.

III. This conversion reduces ferredoxin and act as electron donor.

IV. This conversion requires Mo and FAD as cofactor.

Choose the correct option;

  1. I only 
  2. I, II and IV 
  3. I & III 
  4. None 

7. Which statement is correct about nitrate reductase?

  1. Nitrate reductase is very sensitive towards oxygen. 
  2. FADH2 act as an electron donor. 
  3. FAD, Mo and haem act as prosthetic group. 
  4. It catalyses conversion of NO2- to NH4+. 

8. Plants assimilate nitrate by?

  1. Roots 
  2. Shoots 
  3. Both 
  4. None 

9. Three statements are given about nitrogenase complex;

I) Ferredoxin reduces fe protein.

II) Fe protein reduces Mo-fe protein

III) Mo-fe protein reduce nitrogen

Which statement is correct?

  1. Only I 
  2. Only II 
  3. I, II and III 
  4. None 

10. Which statement is not correct about nitrogen fixation?

  1. Nitrogenase complex catalyse the reduction of nitrogen. 
  2. It requires metabolic energy. 
  3. This process is carried out by only few organisms known as Diazotroph. 
  4. None


  1. Ans. B. 

 The given pairs represent the symbiotic association of plant and nitrogen-fixing microorganisms, in which Frankia is symbiotically associated with Casuarina; Anabaena with free-floating Azolla plant and Brady rhizobium with Soyabean.

  1. Ans. B. 

 In soil, by the addition of nitrogen-containing fertilizer gas exchange process is affected, this type of addition results in the release of NO and N2O.

  1. Ans. A. 

In the process of nitrogen metabolism, conversion of atmospheric nitrogen into ammonia is known as nitrogen fixation;

N2(atmospheriC. + 8 H+ + 8 e → 2 NH3 + H2

and conversion of organic nitrogen into ammonia is called as ammonification.

The process of conversion of nitrite or nitrate into atmospheric nitrogen is known as denitrification; NO32—(Nitrate)-   NO22—(Nitrite) NO (Nitric oxide)  N2O(Nitrous oxide)  N2(atmospheric)

and conversion of ammonium ion into nitrite or nitrate is termed nitrification.

NH3 + 1.5 O2 --> NO2- + H+ + H20

  1. Ans. A. 

 Leghaemoglobin is red coloured pigment which is found in root nodules of legume plants it combines with oxygen and helps in oxygen removal from nodules. Growth of nodule in legumes is regulated by chemical signals between plant and symbiotic microbes. Concentration of NO (nitric oxide) regulates the denitrogenase activity. Expression of nif gene is induced by a response of low oxygen and fixed nitrogen concentration and it is regulated by operon of microbes.

  1. Ans. A. 

During biological nitrogen fixation, 8 ATPs are required for the conversation of one nitrogen into one ammonium ion so in the formation of two ammonium ions, 16 ATP is required.



  1. Ans. B. 

In biological nitrogen fixation conversion of NO3 - into NO2- which takes place in plants, this conversion requires Mo and FAD as cofactor, and it is catalysed by nitrate reductase and this process occurs in the cytosol.

  1. Ans. C. 

FAD, Mo and haem act as prosthetic group to nitrate reductase enzyme in higher plants.

  1. Ans. C. 

Plants known as cocklebur in which nitrogen metabolism is restricted to shoot, or in the white lupin; plant nitrate is metabolised in shoot so nitrate is assimilated by root and shoot both.

  1. Ans. C. 

In the reaction catalysed by nitrogenase complex in plants, ferredoxin reduces Fe protein. Binding and hydrolysis of ATP to fe protein causes confirmational change of fe protein which facilitate the redox reaction. Fe protein reduce Mo-fe protein and Mo-fe protein reduces the nitrogen.

  1. Ans. D. 

 In the process of nitrogen fixation, reduction of atmospheric nitrogen is facilitated by nitrogenase complex, this process is carried out by nitrogen fixing microbes known as Diazotrophs and the process requires metabolic energy.

Download Important Questions on Nitrogen Metabolism in Plants - Download PDF Here


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