CSIR NET 2024 | Life Science: Methods in Biology | 4 April

Over expression of myc is usually associated with human cancers. Which assay is more sensitive to quantitate expression of myc at RNA level in tumor cells vs. normal cells?

Dye used to stain in microscopic and flow cytometry application because of its affinity for GC-rich region of DNA strand is

Which one of the following DNA markers can be used to distinguish between a homozygote and a heterozygote?

Cluster analysis in DNA microarray experiments refers to:

In an experiment designed to demonstrate the feasibility of gene therapy for cancer, cells called tumor infiltrating lymphocytes (TILs) were isolated from a cancer patient. (TILs are T cells that attack tumor cells.) These cells were transfected using a retrovirus vector that contained a neomycin resistance gene. The transfected cells were returned to the patient. After several weeks, the researchers wanted to see if the neomycin resistance gene could still be detected in this patient.
 
If you were doing this experiment, which of the following methods would you use to detect the recombinant neomycin resistance gene in the TIL cells?