Gene therapy is an experimental technique that uses genes to treat or prevent disease. In this technique, functional gene is inserted into target cell should:

Transgenic mouse can be produced by the transfection of ES cells derived from ICM of blastocyst. In an experiment the transgene is injected into the male pronucleus just after the fertilization. The surviving eggs were implanted into a host mouse, the pups obtained were not completely transgenic. What could be the possible reason .

Match the following gene therapy transfer techniques

A) Altered genetically in cell culture in vitro and - (i) In-situ gene therapy

reintroduced back into the patients.

B) Cells treated inside the patient’s body. - (ii) Ex-vivo gene therapy

C) Gene therapy is done in a restricted area - (iii) In-vivo gene therapy

MALDI-TOF is a type of spectrometry. Following are some of the statements made regarding MALDI-TOF. Which of them is/are incorrect?

A) MALDI-TOF is used for analysing protein-protein interactions

B) Lyophilised samples aren’t suitable for MALDI-TOF

C) MALDI-TOF is greatly useful in identifying microorganisms like bacteria and fungi.

D) Samples are coated onto a matrix in MALDI-TOF

Fluorescence microscopy is a microscopy technique used to observe molecules in a cell. Following are some statements made regarding different types of fluorescence microscopy. Identify the incorrect statement.