Which one of the following statements regarding restriction/modifying enzymes used in recombinant DNA technology is correct?

Transgenic mouse can be produced by the transfection of ES cells derived from ICM of blastocyst. In an experiment the transgene is injected into the male pronucleus just after the fertilization. The surviving eggs were implanted into a host mouse, the pups obtained were not completely transgenic. What could be the possible reason .

Which one of the following statements regarding crop improvement programs using molecular breeding approaches is INCORRECT?

Agrobacterium Ti plasmid vectors are used to generate transgenic plants. The following are examples of vir gene-encoded proteins that are important for the transfer of T-DNA into plants:

a. Vir E, a single-stranded DNA binding protein

b. Vir D2 that generates T-strands

c. Vir A that senses plant phenolic compounds

d. Vir F which directs T-complex proteins for destruction in proteasomes

Which one of the following combinations of proteins functions inside the plant cells?

Gene therapy is an experimental technique that uses genes to treat or prevent disease. In this technique, functional gene is inserted into target cell should: