CSIR NET 2022 | Life Science : (Methods in Biology) | (26 January)

SUP4 and URA3 is present in which of the following plasmids?

A molecular biologist analysed 2 bacterial plasmids. Here first digested Plasmid A and Plasmid B with BamHI and analysed for agarose gel electrophoresis. If plasmid A gave two fragments and plasmid B gave three fragments, then which of the following inferences are correct?

P. Plasmid A has three sites and is circular

Q. Plasmid B has three sites and is circular

R. Plasmid A has two sites and is linear

S. Plasmid B has two sites and is linear

Which of the following vectors are used for cloning in insects?

Identification of cells containing vector molecule requires presence of suitable marker on the vector molecule. Following antibiotic selectable marker ‘X’ was used in gene cloning experiment .Function of ‘X’ is it as an irreversible inhibitor of the enzyme transpeptidase, which is needed by bacteria to make the cell wall. Identify X?

Which of the following is not a component of BAC?